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KMID : 1134120210240050463
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Journal of Breast Cancer
2021 Volume.24 No. 5 p.463 ~ p.473
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Longitudinal Intravital Imaging of Tumor-Infiltrating Lymphocyte Motility in Breast Cancer Models
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Park In-Won
Hong Su-Jung
Seok Joon
Lucia Stephani Edwina
Song Eun-Joo
Kim Min-Gyo
Kong Eun-Ji
Seo Ho-Won
Hwang Yoon-Ha
Ahn So-Yeon
Kim Seong-Hye
Jang Dong-Hyun
Lee Jae-Hyuk
Park Su-Hyung
Kim Pil-Han
Jo You-Hwan
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Abstract
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Immunoreactive dynamics of tumor-infiltrating lymphocytes (TILs) within the tumor microenvironment in breast cancer are not well understood. This study aimed to investigate the spatiotemporal cellular dynamics of TILs in breast cancer models. Breast cancer cells were implanted into the dorsal skinfold chamber of BALB/c nude mice, and T lymphocytes were adoptively transferred. Longitudinal intravital imaging was performed, and the spatiotemporal dynamics of TILs were assessed. In the 4T1 model, TILs progressively exhibited increased motility, and their motility inside the tumor was significantly higher than that outside the tumor. In the MDA-MB-231 model, the motility of TILs progressively decreased after an initial increase. TIL motility in the MDA-MB-231 and MCF-7 models differed significantly, suggesting an association between programmed death-ligand 1 expression levels and TIL motility, which warrants further investigation. Furthermore, intravital imaging of TILs can be a useful method for addressing dynamic interactions between TILs and breast cancer cells.
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KEYWORD
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Breast neoplasms, Cell movement, Intravital microscopy, Lymphocytes, tumor-infiltrating, Programmed cell death 1 receptor
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